Title: In-Situ Enzymatic Remodeling of Bacterial Cellulose Architecture Using Cellulase for Scaffold Improvement
1Cellulose for Future Materials and Technologies Special Research Unit, Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, 50 Ngamwongwan Road Chatuchak, Bangkok 10900, Thailand
2Center for Advanced Studies of Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok 10900, Thailand
Bacterial cellulose (BC) is highly valued for its exceptional physical and biological properties. However, its application in tissue engineering remains limited due to inadequate cell adhesion and proliferation. This study aims to investigate the effect of cellulase during BC cultivation and improve its properties, particularly in cell adhesion and proliferation, which are currently insufficient for its application in tissue engineering. The structure and surface morphology were analyze using Scanning Electron Microscopy (SEM). Mechanical properties of scaffold were anlyze with compressive stress using Universal Tensile Machine (UTM). Cytotoxicity and cell proliferation were evaluated using 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetra-zolium (MTS assay) to assess cell viability. BC modified with cellulase exhibited a decreasing of mechanical properties from 0.0635, 0.0224 and 0.0072 MPa, while swelling ratio increasing of 2,540, 3,342 and 4,382% in BC control, BC 0.03 and BC 0.06 respectively. The porosity of scaffold showed a increasing from 92.2241, 93.8395 and 94.7909 of BC control, BC 0.03 and BC 0.06. SEM analysis revealed a progressively loosened fiber structure with increasing cellulase concentration. Cytotoxicity analysis indicating that the cell viability above 70% in both of BC control and cellulase treated samples, demonstrating that the modified BC scaffold provide a more favorable environmental for cell growth. This research advances biotechnology by BC modified with cellulase, to improve its cell adhesion and proliferation properties for potential application in tissue engineering. Furthermore, it is expected to serve as a foundation for future research and studies.
Biography of the presenting author
Mr. Suppanut Sripratum has completed his Bachelor of Science in Biotechnology at the age of 22 years from Kasetsart University. He is currently pursuing a Master of Science in Biotechnology at Kasetsart University, where he continues to develop his research expertise and academic experience in the field.
Presenting author details
Full name: Suppanut Sripratum
Contact number: 0617789105
Email: suppanut.sr@ku..th
LinkedIn: Suppanut Sripratum
number: Medical and Pharmaceutical Biotechnology
Category: Oral presentation
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