Master of Science
Thanh-Tan Nguyen
Laboratory of Biosensors, Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh City, Vietnam
Title: Characterization of Toll-like Receptor 22 in striped catfish (Pangasianodon hypophthalmus): Recognition of Gram-Positive and Gram-Negative bacteria
Name(s): Thanh-Tan Nguyen a,c, Hieu Tran-Van a,b,c*
a Laboratory of Biosensors, Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh City, Vietnam
b Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh City, Vietnam
c Vietnam National University, Ho Chi Minh City, Vietnam
Toll-like receptor 22 (TLR22) in Pangasianodon hypophthalmus is key component of the fish innate immune system, responsible for recognizing pathogen-associated molecular patterns (PAMPs). However, its bacterial recognition mechanisms remain largely unexplored, particularly in striped catfish. In this study, four recombinant subunits of TLR22 – TLR22(LRR1-17), TLR22(LRR1-13), TLR22(LRR1-10), and TLR22(LRR1-4) – were successfully produced in Escherichia coli SHuffle® T7 Express, and protein expression was confirmed via SDS-PAGE and Western blot analysis. Dot blot and ELISA assays were employed to evaluate bacterial-binding activity against Escherichia coli, Aeromonas hydrophila, Streptococcus agalactiae, and Lactiplantibacillus plantarum. Among the subunits, TLR22 (LRR1–4) exhibited the strongest binding affinity, particularly toward Gram-positive bacteria. Furthermore, pre-blocking bacterial peptidoglycan with mouse serum significantly reduced TLR22(LRR1–4) binding, indicating a likely peptidoglycan-mediated interaction. These findings provide the first experimental evidence that striped catfish TLR22 can recognize both Gram-negative and Gram-positive bacteria, broadening current understanding of its ligand recognition spectrum. This study highlights the potential role of TLR22 in bacterial immune detection and establishes a foundations for further investigations into the molecular mechanisms of fish innate immunity. (Up to 300 words)
Biography of the presenting author
I hold a Master’s degree in Microbiology from the University of Science, Vietnam National University Ho Chi Minh City (VNU-HCMC). I am currently a researcher at the Laboratory of Biosensors, Faculty of Biology-Biotechnology, University of Science, VNU-HCMC. I have more than five years of experience in the research and application of recombinant proteins in biotechnology, particularly in the health-related sector. To date, I have authored over eleven scientific publications, including five articles published in reputable international journals indexed in Scopus/Web of Science (one Q2, one Q3, and three Q4 papers), four domestic journal articles, and two papers in national conference proceedings. Further research experience was gained through a six-month placement at the Nanobiomaterials Application Laboratory, National Cheng Kung University, Taiwan, focusing on biosensor development, and a two-month research visit at the University of Natural Resources and Life Sciences (BOKU), Austria, centered on recombinant protein studies. (Up to 200 words)
Presenting author details
Full name: Thanh-Tan Nguyen
Contact number: (+84) 903183198
Email: nttan@hcmus.edu.vn
ResearcherID:
ORCID: 0000-0001-6305-0468
Session name/ number: Aquaculture and Animal Biotechnology
Category: Oral presentation