Professor
Dewi Sukma
Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia
Flavonol Abundance in Early Flower Stage of French Marigold (Tagetes patula): A Metabolomic Study
Shania Nur Fajrinaa, Muhamad Syukura,b, Syarifah Iis Aisyaha,b, Deden Derajat Matrab, Dewa Ngurah Supraptac, Waras Nurcholisd, Ming-Tsair Chane, and Dewi Sukmaa,b*
a Plant Breeding and Biotechnology Graduate Program, Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Jl. Meranti, Kampus IPB Darmaga, Bogor 16680, West Java, Indonesia.
b Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Jl. Meranti, Kampus IPB Darmaga, Bogor 16680, West Java, Indonesia.
c Faculty of Agriculture, Udayana University, Jl. P.B. Sudirman, Dauh Puri Klod, Denpasar Bar., Kota Denpasar 80234, Bali, Indonesia
d Department of Biochemistry, Faculty of Mathematics and Natural Sciences, IPB University, Bogor, 16680, Indonesia.
e Academia Sinica Biotechnology Center in Southern Taiwan, Agricultural Biotechnology Research Center, Tainan City 71101, Taiwan (R.O.C.).
* Corresponding author: Dewi Sukma. orcid.org/0000-0003-3026-8182. E-mail: dewi_sukma@apps.ipb.ac.id
Abstract
French marigolds (Tagetes patula) have been recognized for their rich flavonoid content, which demonstrates significant biological activities, such as antioxidant and anti-inflammatory properties. Despite their phytochemical potential, limited information exists regarding the dynamic changes in flavonoid content across various flower developmental stages. This study explores the dynamic changes in flavonoid content across four developmental stages of Tagetes patula cv. Sudamala Barak marigold flowers, aiming to optimize metabolite extraction and enhance understanding of biochemical mechanisms. The stages include closed flower buds (Stage 1, S1), partially opened buds (Stage 2, S2), fully opened flowers (Stage 3, S3), and mature flowers with strong orange coloration (Stage 4, S4). Petals were extracted using 70% methanol containing 0.1% HCl for analysis. Metabolite profiling was conducted using LC-HRMS, identifying 93 compounds across the stages. Notably, the early developmental stage (S1) exhibited a high abundance of flavonol compounds such as patuletin, luteolin, isorhamnetin, and myricetin. These findings offer valuable insights into the biochemical profiles of marigold flowers, facilitating deeper exploration of their phytochemical potential and supporting advancements in the utilization of high-value flavonoid compounds.
Keywords: Flavonol derived compounds, Flower development stages, French marigold, LC-HRMS, Metabolite profiling
Biography of the presenting author
Dewi Sukma is a Lecturer at IPB University, Indonesia, and a member of the Plant Biotechnology Division, Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University (IPB University), Dramaga Campus, Bogor 16680, Indonesia. She currently serves as Head of the Master’s Program in Plant Breeding and Biotechnology at the Faculty of Agriculture, IPB University, and as Chief Editor of the Indonesian Journal of Horticulture.
At IPB University, she teaches a wide range of courses including Principles of Agronomy, Horticulture, Plant Propagation, Ornamental Plants, Biotechnology in Plant Breeding, Plant Growth Regulators, and Plant Growth and Developmental Regulation.
Her research focuses on plant tissue culture propagation, biotechnology approaches in plant breeding, biochemical, cellular, and molecular plant analysis, molecular marker development, plant disease resistance mechanisms, and genetic transformation. Much of her work emphasizes ornamental plants, particularly orchids and marigolds, with the aim of advancing breeding strategies and enhancing genetic improvement in horticultural crops.
Presenting author details
Full name: Dewi Sukma
Contact number:62-85280147350
Email: dewi_sukma@apps.ipb.ac.id
Session name/ number: Agricultural Biotechnology
Category: (Oral presentation/ Poster presentation)