Professor
Xinhui Xing
Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, China
Title: AI-empowered discovery of bioactive peptides as glucose homeostasis modulator capable of targeting DPP-IV and gut microbiota
Discovery of new dipeptidyl peptidase IV (DPP-IV) inhibitory peptides from natural protein resources capable of regulating glucose metabolism in type 2 diabetic populations has been a significant challenge. In this study, we constructed a molecular docking- and machine learning-aided DPP-IV inhibitory peptide library and combined a functional screening approach based on intestinal organoids to discover efficient and new DPP-IV-inhibiting peptides from hemp seed protein hydrolysates. A novel tetrapeptide, VAMP, was then identified to strongly inhibit DPP-IV (IC50=1.00 μM in vitro), which competitively binds to DPP-IV and improves glucose metabolism in vivo with high safety by increasing active glucagon-like peptide-1 (GLP-1) levels in obese mouse models. Interestingly, VAMP specifically promoted the growth and abundance of intestinal Akkermansia muciniphila in vivo, at the same time, which was responsible for the improved intestinal barrier function and insulin resistance. Our study demonstrated that the novel bifunctional VAMP can effectively target the DPP-IV-GLP-1 axis and simultaneously regulate the abundance of the gut microbial A. muciniphila, to regulate glucose homeostasis, providing a promising nutraceutical and therapeutic tetrapeptide for hyperglycaemia treatment by targeting the gut-microbiata axis.
Biography of the presenting author
Prof. Xin-Hui XING received his Ph.D. from Tokyo Institute of Technology in 1992. He had been the Assistant Professor at Tokyo Institute of Technology from 1992 to 1998, and Associate Professor at Yokohama National University from 1998 to2001. He was selected as a full professor by the 100-Telent Scholar Program of Tsinghua University in 2000 and joined Department of Chemical Engineering since then. He had served the director of Institute of Biochemical Engineering from 2002 to 2022. He had been the vice department chairman from 2009 to 2018. From 2019, he has being served the Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School as the Deputy Dean. His research field covers enzyme engineering, high throughput biobreeding technology and instrumentation, synthetic biotechnology, health engineering, glycodrug technology, bioactive peptide mining and biomanufacturing. He serves as Editor-in-Chief of Health Engineering, Associate Editor of Biochemical Engineering Journal, and editorial board of more than 10 domestic and international journals. He has filed more than 100 invention patents and 20 of them were licensed for industrial application. He has cofounded 3 venture companies for transforming research. In recent years, he has received 10 research awards and honors.
Presenting author details
Full name: Xinhui Xing
Contact number:86+13901324869
Email: xhxing@mail.tsinghua.edu.cn
ORCID: 0000-0001-6419-4770
Category: (Oral presentation/ Poster presentation)