Professor
Chanpen Chanchao
Department of Biology, Faculty of Science, Chulalongkorn University, 254 Phaya Thai Rd., Patumwan, Bangkok, 10330 Thailand
Title: Potential of Thai Tetragonula laeviceps propolis in inducing program cell death and cell arrest in liver cancer cells
Chanpen Chanchao1, Thitipan Meemongkolkiat1,2, Phanthiwa Khongkarat1, Songchan Puthong3, Preecha Rod-im4, Orawan Duangphakdee4, Packapong Tuthaisong5, Preecha Phuwapraisirisan5
1Department of Biology (Faculty of Science), 3Institute of Biotechnology and Genetic Engineering, and 5Department of Chemistry (Faculty of Science), Chulalongkorn University, Bangkok, Thailand
2Department of Medical Sciences, Faculty of Science, Rangsit University, Pathum Thani, Thailand
4Native Honeybee and Pollinator Research Center, Ratchaburi Campus, King Mongkut’s University of Technology Thonburi, Ratchaburi, Thailand
Propolis is one of economic bee products that have long been used in traditional medicine in Thailand. Crude extracts, partial purified forms, or pure compounds in propolis have been reported to be potential in many bioactivities. Here, the antiproliferation of cancer cells, especially liver cancer cells is focused since liver cancer, especially hepatocellular carcinoma, is one of the leading causes of cancer-related deaths in Thailand. Also, treatment options are often limited by high recurrence rates and severe side effects. The potential of mangiferolic acid (MF) which could be isolated from stingless bee (Tetragonula laeviceps) propolis in Ratchaburi province, Thailand is stressed as an alternative compound for Hep-G2 liver cancer inhibition. Overall, MF showed a strong cytotoxicity against Hep-G2 liver cancer cells, with IC50 values between 8.35 – 18.41 µg/mL. Morphological analysis by a compound microscope showed MF-induced cell shrinkage and density reduction in a dose- and time-dependent manner. Cell staining with annexin-V and propidium iodide (PI) and flow cytometry analysis presented apoptosis induction, with significant increases in late apoptotic and necrotic cells after 72 h at 30 µg/mL. Furthermore, by cell staining with PI and flow cytometry analysis, MF also caused G1-phase cell cycle arrest, with up to 70.24% of cells halted in the G1 phase compared to 57.50% in the control, leading to blocking the progression to the S phase. These results highlighted the ability of MF to inhibit Hep-G2 cancer cell proliferation. However, more research is needed to fully understand its mechanisms and therapeutic potential.
Biography of the presenting author
Miss Chanpen Chanchao has completed her PhD from Virginia Tech (Blacksburg, USA) and research fellow from National Institute of Livestock and Grassland Science (Tsukuba, Japan) and Hokkaido University (Sapporo, Japan). She is a professor in Department of Biology, Faculty of Science, Chulalongkorn University (Bangkok, Thailand). Her research is focused on bee and bee products at the cellular and molecular level.
Presenting author details
Full name: Professor Dr. Chanpen Chanchao
Contact number: +6685 913 0412
Email: chanpen.c@chula.ac.th
Scopus ID: 6506947616
Web of Science Researcher ID: ITV-4731-2023
Google Scholar ID: X9-BqRIAAAAJ
Session name/ number: Agricultural Biotechnology/ 2
Category: Oral presentation